{"id":15344,"date":"2026-03-13T21:42:20","date_gmt":"2026-03-13T20:42:20","guid":{"rendered":"https:\/\/www.future-live.com\/biofilm-and-proteolytic-enzymes-a-targeted-approach-to-persistent-infections\/"},"modified":"2026-03-13T21:42:22","modified_gmt":"2026-03-13T20:42:22","slug":"biofilm-and-proteolytic-enzymes-a-targeted-approach-to-persistent-infections","status":"publish","type":"post","link":"https:\/\/www.future-live.com\/en\/biofilm-and-proteolytic-enzymes-a-targeted-approach-to-persistent-infections\/","title":{"rendered":"Biofilm and proteolytic enzymes: a targeted approach to persistent infections"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text]In persistent or recurring infections, the problem is not only the presence of the microorganism but also the way it manages to survive within the body.<\/p>\n<p>Biofilm represents a fundamental protection strategy for many bacteria and yeasts. As long as this structure remains intact, even correct therapies may be only partially effective. <\/p>\n<p>For this reason, in recent years, research focus has shifted toward approaches capable of acting not only on the microorganism but also on the structure that protects it.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text]<\/p>\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_65 ez-toc-wrap-center counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title \" >Indice<\/p>\n<span class=\"ez-toc-title-toggle\"><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/www.future-live.com\/en\/biofilm-and-proteolytic-enzymes-a-targeted-approach-to-persistent-infections\/#Why_biofilm_is_a_therapeutic_target\" title=\"Why biofilm is a therapeutic target\">Why biofilm is a therapeutic target<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/www.future-live.com\/en\/biofilm-and-proteolytic-enzymes-a-targeted-approach-to-persistent-infections\/#What_are_proteolytic_enzymes\" title=\"What are proteolytic enzymes\">What are proteolytic enzymes<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/www.future-live.com\/en\/biofilm-and-proteolytic-enzymes-a-targeted-approach-to-persistent-infections\/#Proteolytic_enzymes_and_biofilm_the_scientific_rationale\" title=\"Proteolytic enzymes and biofilm: the scientific rationale\">Proteolytic enzymes and biofilm: the scientific rationale<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/www.future-live.com\/en\/biofilm-and-proteolytic-enzymes-a-targeted-approach-to-persistent-infections\/#Patented_enzymatic_blend\" title=\"Patented enzymatic blend\">Patented enzymatic blend<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/www.future-live.com\/en\/biofilm-and-proteolytic-enzymes-a-targeted-approach-to-persistent-infections\/#Quando_considerare_un_approccio_mirato_al_biofilm\" title=\"Quando considerare un approccio mirato al biofilm\">Quando considerare un approccio mirato al biofilm<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/www.future-live.com\/en\/biofilm-and-proteolytic-enzymes-a-targeted-approach-to-persistent-infections\/#A_shift_in_perspective_for_managing_persistent_infections\" title=\"A shift in perspective for managing persistent infections\">A shift in perspective for managing persistent infections<\/a><\/li><\/ul><\/nav><\/div>\n<h2><span class=\"ez-toc-section\" id=\"Why_biofilm_is_a_therapeutic_target\"><\/span>Why biofilm is a therapeutic target<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>[\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]The biofilm is a protective structure produced by bacteria and released externally; it thus appears as an extracellular matrix that encloses and protects them.<\/p>\n<p>This matrix is mainly composed of:<\/p>\n<ul>\n<li>polysaccharides<\/li>\n<li>proteins<\/li>\n<li>extracellular DNA<\/li>\n<\/ul>\n<p>The presence of protein components makes the biofilm particularly stable and resistant to the action of external agents.<\/p>\n<p>Acting on the protein component of the biofilm therefore represents one of the strategies to undermine its overall structure.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text]<\/p>\n<h2><span class=\"ez-toc-section\" id=\"What_are_proteolytic_enzymes\"><\/span>What are proteolytic enzymes<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>[\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]Proteolytic enzymes are molecules capable of degrading proteins, breaking them down into simpler components.<\/p>\n<p>In the biological field, these types of enzymes are involved in numerous physiological processes. In relation to biofilm, the interest lies in the possibility of intervening on the protein components of the extracellular matrix. <\/p>\n<p>Reducing the integrity of the matrix can make the biofilm less compact and more vulnerable.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text]<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Proteolytic_enzymes_and_biofilm_the_scientific_rationale\"><\/span>Proteolytic enzymes and biofilm: the scientific rationale<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>[\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]From a scientific perspective, the use of proteolytic enzymes is based on a key principle: weakening the structure of the biofilm to reduce the protection of microorganisms.<\/p>\n<p>When the extracellular matrix is partially broken down:<\/p>\n<ul>\n<li>bacteria are less protected<\/li>\n<li>the permeability of the biofilm increases<\/li>\n<li>the risk of infection persistence can be reduced<\/li>\n<\/ul>\n<p>This approach does not replace traditional therapies but acts as a complementary strategy in contexts where biofilm plays a central role.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text]<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Patented_enzymatic_blend\"><\/span>Patented enzymatic blend<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>[\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]A specific blend of proteolytic enzymes consisting of <a href=\"https:\/\/www.future-live.com\/en\/bromelain\/\"><strong>Bromelain<\/strong><\/a>, extracted from the pineapple stem, <a href=\"https:\/\/www.future-live.com\/en\/papain\/\"><strong>Papain<\/strong><\/a>, derived from papaya, and <a href=\"https:\/\/www.future-live.com\/en\/fungal-protease\/\"><strong>Fungal Protease<\/strong><\/a> extracted from the <em>Aspergillus oryzae<\/em> fungus, was developed to act on the structure of the biofilm.<\/p>\n<p>The formulation has been patented and was evaluated through an in vitro study that analyzed its activity on <em>Escherichia coli<\/em> biofilm.<\/p>\n<p>The results showed the enzymatic blend&#8217;s ability to limit biofilm formation and break down its matrix, confirming the rationale of the enzymatic approach.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text]<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Quando_considerare_un_approccio_mirato_al_biofilm\"><\/span>Quando considerare un approccio mirato al biofilm<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>[\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]An intervention that takes into account the biofilm can be particularly relevant in the presence of:<\/p>\n<ul>\n<li>recurrent cystitis<\/li>\n<li>bacterial prostatitis<\/li>\n<li>persistent urinary tract infections<\/li>\n<li>disorders that improve only temporarily after therapy<\/li>\n<\/ul>\n<p>In these contexts, the biofilm can represent one of the factors contributing to the chronicization of the problem.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text]<\/p>\n<h2><span class=\"ez-toc-section\" id=\"A_shift_in_perspective_for_managing_persistent_infections\"><\/span>A shift in perspective for managing persistent infections<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>[\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]Addressing the biofilm means going beyond the management of a single infectious episode and considering the mechanisms that allow microorganisms to survive over time.<br \/>\nThe enzymatic approach represents one of the most studied strategies for intervening on the protective structure of the biofilm, opening new possibilities in the management of recurrent infections.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"[vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text]In persistent or recurring infections, the problem is not only the presence of the microorganism but also the way it manages to survive within the body. Biofilm represents a fundamental protection strategy for many bacteria and yeasts. As long as this structure remains intact, even correct therapies may be only partially effective. For this reason, in recent years, research focus has shifted toward approaches capable of acting not only on the microorganism but also on the structure that protects it.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] Why biofilm is a therapeutic target [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]The biofilm is a protective structure produced by bacteria and released externally; it thus appears as an extracellular matrix that encloses and protects them. This matrix is mainly composed of: polysaccharides proteins extracellular DNA The presence of protein components makes the biofilm particularly stable and resistant to the action of external agents. Acting on the protein component of the biofilm therefore represents one of the strategies to undermine its overall structure.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] What are proteolytic enzymes [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]Proteolytic enzymes are molecules capable of degrading proteins, breaking them down into simpler components. In the biological field, these types of enzymes are involved in numerous physiological processes. In relation to biofilm, the interest lies in the possibility of intervening on the protein components of the extracellular matrix. Reducing the integrity of the matrix can make the biofilm less compact and more vulnerable.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] Proteolytic enzymes and biofilm: the scientific rationale [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]From a scientific perspective, the use of proteolytic enzymes is based on a key principle: weakening the structure of the biofilm to reduce the protection of microorganisms. When the extracellular matrix is partially broken down: bacteria are less protected the permeability of the biofilm increases the risk of infection persistence can be reduced This approach does not replace traditional therapies but acts as a complementary strategy in contexts where biofilm plays a central role.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] Patented enzymatic blend [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]A specific blend of proteolytic enzymes consisting of Bromelain, extracted from the pineapple stem, Papain, derived from papaya, and Fungal Protease extracted from the Aspergillus oryzae fungus, was developed to act on the structure of the biofilm. The formulation has been patented and was evaluated through an in vitro study that analyzed its activity on Escherichia coli biofilm. The results showed the enzymatic blend&#8217;s ability to limit biofilm formation and break down its matrix, confirming the rationale of the enzymatic approach.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] Quando considerare un approccio mirato al biofilm [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]An intervention that takes into account the biofilm can be particularly relevant in the presence of: recurrent cystitis bacterial prostatitis persistent urinary tract infections disorders that improve only temporarily after therapy In these contexts, the biofilm can represent one of the factors contributing to the chronicization of the problem.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] A shift in perspective for managing persistent infections [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]Addressing the biofilm means going beyond the management of a single infectious episode and considering the mechanisms that allow microorganisms to survive over time. The enzymatic approach represents one of the most studied strategies for intervening on the protective structure of the biofilm, opening new possibilities in the management of recurrent infections.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row]","protected":false},"author":178,"featured_media":14954,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[156,165,162,151],"tags":[173],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Biofilm and proteolytic enzymes: a targeted approach to persistent infections - Future Live Srl<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.future-live.com\/en\/biofilm-and-proteolytic-enzymes-a-targeted-approach-to-persistent-infections\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Biofilm and proteolytic enzymes: a targeted approach to persistent infections - Future Live Srl\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text]In persistent or recurring infections, the problem is not only the presence of the microorganism but also the way it manages to survive within the body. Biofilm represents a fundamental protection strategy for many bacteria and yeasts. As long as this structure remains intact, even correct therapies may be only partially effective. For this reason, in recent years, research focus has shifted toward approaches capable of acting not only on the microorganism but also on the structure that protects it.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] Why biofilm is a therapeutic target [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]The biofilm is a protective structure produced by bacteria and released externally; it thus appears as an extracellular matrix that encloses and protects them. This matrix is mainly composed of: polysaccharides proteins extracellular DNA The presence of protein components makes the biofilm particularly stable and resistant to the action of external agents. Acting on the protein component of the biofilm therefore represents one of the strategies to undermine its overall structure.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] What are proteolytic enzymes [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]Proteolytic enzymes are molecules capable of degrading proteins, breaking them down into simpler components. In the biological field, these types of enzymes are involved in numerous physiological processes. In relation to biofilm, the interest lies in the possibility of intervening on the protein components of the extracellular matrix. Reducing the integrity of the matrix can make the biofilm less compact and more vulnerable.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] Proteolytic enzymes and biofilm: the scientific rationale [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]From a scientific perspective, the use of proteolytic enzymes is based on a key principle: weakening the structure of the biofilm to reduce the protection of microorganisms. When the extracellular matrix is partially broken down: bacteria are less protected the permeability of the biofilm increases the risk of infection persistence can be reduced This approach does not replace traditional therapies but acts as a complementary strategy in contexts where biofilm plays a central role.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] Patented enzymatic blend [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]A specific blend of proteolytic enzymes consisting of Bromelain, extracted from the pineapple stem, Papain, derived from papaya, and Fungal Protease extracted from the Aspergillus oryzae fungus, was developed to act on the structure of the biofilm. The formulation has been patented and was evaluated through an in vitro study that analyzed its activity on Escherichia coli biofilm. The results showed the enzymatic blend&#8217;s ability to limit biofilm formation and break down its matrix, confirming the rationale of the enzymatic approach.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] Quando considerare un approccio mirato al biofilm [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]An intervention that takes into account the biofilm can be particularly relevant in the presence of: recurrent cystitis bacterial prostatitis persistent urinary tract infections disorders that improve only temporarily after therapy In these contexts, the biofilm can represent one of the factors contributing to the chronicization of the problem.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] A shift in perspective for managing persistent infections [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]Addressing the biofilm means going beyond the management of a single infectious episode and considering the mechanisms that allow microorganisms to survive over time. 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Future Live Srl","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.future-live.com\/en\/biofilm-and-proteolytic-enzymes-a-targeted-approach-to-persistent-infections\/","og_locale":"en_US","og_type":"article","og_title":"Biofilm and proteolytic enzymes: a targeted approach to persistent infections - Future Live Srl","og_description":"[vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text]In persistent or recurring infections, the problem is not only the presence of the microorganism but also the way it manages to survive within the body. Biofilm represents a fundamental protection strategy for many bacteria and yeasts. As long as this structure remains intact, even correct therapies may be only partially effective. For this reason, in recent years, research focus has shifted toward approaches capable of acting not only on the microorganism but also on the structure that protects it.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] Why biofilm is a therapeutic target [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]The biofilm is a protective structure produced by bacteria and released externally; it thus appears as an extracellular matrix that encloses and protects them. This matrix is mainly composed of: polysaccharides proteins extracellular DNA The presence of protein components makes the biofilm particularly stable and resistant to the action of external agents. Acting on the protein component of the biofilm therefore represents one of the strategies to undermine its overall structure.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] What are proteolytic enzymes [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]Proteolytic enzymes are molecules capable of degrading proteins, breaking them down into simpler components. In the biological field, these types of enzymes are involved in numerous physiological processes. In relation to biofilm, the interest lies in the possibility of intervening on the protein components of the extracellular matrix. Reducing the integrity of the matrix can make the biofilm less compact and more vulnerable.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] Proteolytic enzymes and biofilm: the scientific rationale [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]From a scientific perspective, the use of proteolytic enzymes is based on a key principle: weakening the structure of the biofilm to reduce the protection of microorganisms. When the extracellular matrix is partially broken down: bacteria are less protected the permeability of the biofilm increases the risk of infection persistence can be reduced This approach does not replace traditional therapies but acts as a complementary strategy in contexts where biofilm plays a central role.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] Patented enzymatic blend [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]A specific blend of proteolytic enzymes consisting of Bromelain, extracted from the pineapple stem, Papain, derived from papaya, and Fungal Protease extracted from the Aspergillus oryzae fungus, was developed to act on the structure of the biofilm. The formulation has been patented and was evaluated through an in vitro study that analyzed its activity on Escherichia coli biofilm. The results showed the enzymatic blend&#8217;s ability to limit biofilm formation and break down its matrix, confirming the rationale of the enzymatic approach.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] Quando considerare un approccio mirato al biofilm [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]An intervention that takes into account the biofilm can be particularly relevant in the presence of: recurrent cystitis bacterial prostatitis persistent urinary tract infections disorders that improve only temporarily after therapy In these contexts, the biofilm can represent one of the factors contributing to the chronicization of the problem.[\/vc_column_text][tm_spacer size=&#8221;lg:30&#8243;][\/vc_column][vc_column width=&#8221;1\/6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/6&#8243;][\/vc_column][vc_column width=&#8221;2\/3&#8243;][vc_column_text] A shift in perspective for managing persistent infections [\/vc_column_text][tm_spacer size=&#8221;lg:10&#8243;][vc_column_text]Addressing the biofilm means going beyond the management of a single infectious episode and considering the mechanisms that allow microorganisms to survive over time. 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